A. Altima HILIC (AH) column; B. Poroshell120 HILIC (PH) column; C. Prevail Carbohydrate (PC) column; and D. Hypercarb (TH) column with the presence of (i) No buffer (NB), (ii) 0.1% formic acid (FA), (iii) 0.1% acetic acid (AA) and (iv) 10mM ammonium formate (AF) in mobile phases.

Figure 3: Retention times of lentinan at 2.5 mg/mL standard concentration