Parameters
Column types Altima HP HILIC
(AH)
Poroshell120 HILIC (PH) Prevail Carbohydrate (PC) Hypercarb (TH)
Particle size (µ) 3 2.7 5 3
Column Size (mm) 2.1 x150 2.1 x 150 4.6 x 150 2.1 x 100
Injection volume (µl) 1 1 1 0.5
Flow rate (mL/min) 0.2 0.3 0.7 0.15
Gradient type Normal phase Normal phase Normal phase Reverse phase
Column temperature (°C) 25 25 30 25
Table 1: Parameters setup for different types of columns (as reported by column packing note)