Column & Buffer Combination
Collision energy (CID), v
Generation of product ions (m/z) at 2.5 mg/mL lentinan
1151
989
827
665
503
341
161
AH;NB
0
√
√
√
√
√
x
x
10
√
√
√
√
x
√
x
20
√
√
√
√
√
√
√
40
x
x
√
√
√
√
√
AH;0.1% FA
0
√
√
√
√
√
x
√
10
√
√
√
√
√
x
x
20
√
√
√
√
√
√
√
40
√
x
√
√
√
√
√
AH;10 mM AF
0
√
√
√
√
x
x
x
10
√
√
√
√
√
√
x
20
√
√
√
√
√
x
√
40
x
x
√
√
√
√
√
AH;0.1% AA
0
√
√
√
√
x
x
x
10
√
√
√
√
√
√
√
20
√
√
√
√
√
√
√
40
x
x
√
√
√
√
√
PH;NB
0
√
√
√
x
x
√
x
10
√
√
√
√
√
x
√
20
√
√
√
√
√
√
√
40
√
x
√
√
√
√
√
PH;0.1% FA
0
√
x
x
x
x
x
x
10
x
x
x
x
x
x
x
20
√
x
x
√
x
x
x
40
x
x
√
√
x
x
x
PH;10 mM AF
0
√
√
√
√
√
x
x
10
√
√
√
√
√
√
√
20
√
√
√
√
√
√
√
40
√
x
√
√
√
√
√
PH;0.1% AA
0
√
√
x
x
x
x
√
10
√
√
√
√
x
x
x
20
√
√
√
√
x
√
x
40
x
x
√
√
x
√
√
PC;NB
0
√
√
√
√
√
√
x
10
√
√
√
√
√
√
√
20
√
√
√
√
√
√
√
40
√
x
√
√
x
√
√
PC;0.1% FA
0
√
√
√
x
x
x
x
10
√
√
√
√
√
x
√
20
√
√
√
√
√
√
√
40
x
x
√
√
√
√
√
PC;10 mM AF
0
x
x
x
x
x
x
x
10
x
x
x
x
x
x
x
20
x
x
x
x
x
x
x
40
x
x
x
x
x
x
x
PC;0.1% AA
0
√
√
√
√
x
x
√
10
√
√
√
√
√
√
√
20
√
√
√
√
√
√
√
40
x
x
√
√
√
√
√
TH;NB
0
√
√
√
√
x
x
x
10
√
√
√
√
x
x
x
20
√
√
√
√
√
√
√
40
x
x
√
√
√
√
√
TH;0.1% FA
0
√
√
√
x
x
√
x
10
√
√
√
√
x
x
x
20
√
√
√
√
√
√
√
40
x
x
√
√
√
√
√
TH;10 mM AF
0
√
√
√
x
x
x
x
10
√
√
√
√
x
√
√
20
√
√
√
√
√
x
x
40
x
x
√
√
√
√
√
TH;0.1% AA
0
√
√
√
x
x
x
x
10
√
√
√
√
x
√
√
20
√
√
√
√
√
√
√
40
√
x
√
√
√
√
√
Table 2:
Summary of product ions of 2.5 mg/mL lentinan generated at different CID levels for various combinations of column and buffer
