Column & Buffer Combination MS/MS spectra generation at different concentration (mg/mL) of lentinan 0.078 0.156 0.312 0.625 1.25 2.5 AH;NB x √ √ √ √ √ AH;0.1% FA x x x √ √ √ AH;10 mM AF x x x x x x AH;0.1% AA x x x √ √ √ PH;NB x x x √ √ √ PH;0.1% FA x x x x x x PH;10 mM AF x x x √ √ √ PH;0.1% AA x x x x x x PC;NB x x √ √ √ √ PC;0.1% FA x x √ √ √ √ PC;10 mM AF x x x x x x PC;0.1% AA x x √ √ √ √ TH;NB x x x x x √ TH;0.1% FA x x √ √ √ √ TH;10 mM AF x x x x x x TH;0.1% AA x √ √ √ √ √

Table 3: MS/MS response (spectra generation of six lentinan product ions) of different lentinan concentrations at 20.0V CID