Herbal formulation Column features Resolution Reference Longdan Xiegan Decoction 1st column: immobilized liposomes 2nd column: RP more than 50 compounds were separated Wang et al., 2009 Rhizoma chuanxiong 1st column: cyano 2nd column: ODS more than 50 compounds were separated Chen et al., 2004 Saponins in extracts of Panax notoginseng 1st column: HILIC 2nd column: RP 224 saponins were found (peak capacity 10200) Xing et al., 2012 Extracts of umbelliferae herbs Ligusticum chuanxiong Hort and Angelica sinensis (Oliv.) Diels 1st column: cyano 2nd column: silica monolithic ODS between 100 and 120 components were efficiently separated in each extract Hu et al., 2005 Flavonol glycosides in the leaves of Maytenus ilicifolia 1st column: size exclusion 2nd column: RP better resolution compared to 1D analysis Souza et al., 2009 Extracts of Psoralea corylifolia 1st column: ion exchange 2nd column: RP more than 188 components were separated Chen et al., 2005 Extract of Rheum palmatum L. 1st column: silica-bonded human serum albumin 2nd column: silica monolithic ODS better resolution compared to 1D analysis Hu et al., 2006

Table 1: Applications of 2D-LC analysis on the comprehensive characterization of herbal formulations.