| Parameter |
Setting |
| LC Method Pump 1 |
Gradient |
| Reservoir A = 10% MeOH in water, 5 mM formic acid |
| Reservoir B = ACN, 5 mM formic acid |
| Time (min) |
%A |
%B |
Flow Rate (µL/min) |
| 0.0 |
93 |
7 |
300 |
| 0.5 |
93 |
7 |
300 |
| 2.0 |
50 |
50 |
300 |
| 3.0 |
50 |
50 |
300 |
| 3.01 |
93 |
7 |
300 |
| 6.0 |
93 |
7 |
300 |
| Column Oven |
Temperature ranges from 40 °C |
| Column Type |
Polar RP Phenyl column, 2x100 mm, 2.5µm particle size |
| Injection Volume |
20 µL |
| Needle Rinse Time |
5 - 10 seconds, using flushport |
| Rinse Solvent |
Mobile Phase A |
| Injection Mode |
Standard |
| Autosampler Tray Temp. |
20 °C |
| Typical Retention Time |
L-Abrine = 2.1 min |
| Ricinine = 3.5 min |
| HPLC Degasser |
Powered for continuous use (there are no settings) |
| MS Scan Mode |
MS/MS selected reaction monitoring (SRM) |
| Ionization Type |
Turbo-ionspray (similar to ESI) |
| Ion Polarity Mode |
Positive ion |
| Scan Type |
100 msec per channel |
| Collision Gas (CAD) |
High or "12" |
| Curtain Gas (CUR) |
20 |
| Ion Source Gas 1 (GS1) |
65 |
| Ion Source Gas 2 (GS2) |
10 |
| Ion Spray Voltage (IS) |
5100 |
| Temperature (TEM) |
550 (interface heater on) |
| Declustering Potential (DP) |
see Table 5 |
| Entrance Potential (EP) |
10 |
| Collision Energy (CE) |
see Table 5 |
