Analyte Linear range,a (ng/g) LOD,b(ng/g) LOQc (ng/g) MDLd (ng/g) Acetaminophen 3.12 - 400 0.30 0.99 4.40 Atenolol 1.25 - 160 0.48 1.62 1.48 Cimetidine 0.625 - 80 0.24 0.81 1.04 Codeine 4.60 - 600 1.07 3.55 6.11 1,7-dimethylxanthine 0.625 - 80 0.17 0.58 1.02 Lincomycin 3.12 - 400 0.63 2.09 5.53 Trimethoprim 1.25 - 160 0.79 2.63 2.15 Thiabendazole 1.25 - 160 0.14 0.47 2.63 Caffeine 3.12 - 400 0.34 1.15 3.93 Sulfamethoxazole 1.25 - 160 0.23 0.76 2.29 Metoprolol 1.25 - 160 0.25 0.85 2.50 Propranolol 0.625 - 80 0.01 0.03 1.07 Diphenhydramine 0.0625 - 8 0.01 0.03 0.05 Diltiazem 0.09 - 12 0.04 0.13 0.12 Carbamazepine 0.625 - 80 0.03 0.12 0.54 Tylosin 3.12 - 400 1.18 3.93 5.02 Fluoxetine 4.69 - 600 0.76 2.54 6.73 Norfluoxetine 3.12 - 400 0.32 1.08 2.90 Sertraline 3.12 - 400 0.21 0.71 3.57 Erythromycin 3.12 - 400 0.85 2.84 6.42 Clofabric acid 1.25 - 160 0.10 0.32 2.69 Warfarin 0.625 - 80 0.09 0.29 0.86 Miconazole 3.12 - 400 0.39 1.32 10.8 Ibuprofen 25 - 3200 3.14 10.4 45.9 Gemfibrozil 3.12 - 400 0.25 0.85 6.68

Table 1: Investigated linear range, LOD, LOQ, and MDL for target analytes in fish muscle tissuea. aclean tissues employed in the determination of these parameters were extracted using a 1:1 mixture of 0.1 M acetic acid (pH 4) and methanol. bLOD, calculated as3 times the standard deviation in the background signal observed for the replicate analysis of a tissue blank. cLOQ, calculated as 10 times the standard deviation in the background signal observed for the replicate analysis of a tissue blank, d MDL, determined by multiplying the one-sided Student’s t–statistics at 99% confidence limit times the standard deviation observed for eight replicate analyses of a matrix spike (spiking level ≤ 10 x MDL). (SOURCE Reproduced with permission from reference 64, Copyright© American Chemical Society).