Exposure time, Day MX exposure MK exposure MX conc. (mg/mL) Fish wet weight, (g) Liver wet weigh, (g) MX dose /trout, (mg) Average dosing level, (mg/g) MK conc. (mg/mL) Fish wet weigh, (g) Liver wet weigh, (g) MK dose /trout, (mg) Average dosing level, (mg/g) 1- Day 10 202 0.75 2.0 0.01 10 257 0.88 2.6 0.01 256 0.87 2.5 237 0.77 2.4 165 0.50 1.6 222† NC 2.2 30 180 0.75 5.4 0.03 30 199 0.70 6.0 0.03 256 0.92 7.5 230 0.76 6.9 280‡ 1.41 8.4 212 0.69 6.3 100 236 0.76 24.0 0.10 100 272 1.26 27.0 0.10 264 0.92 26.0 271 1.26 27.0 204 0.74 20.0 197 0.78 20.0 300 250 0.88 75.0 0.30 300 190 0.70 57.0 0.30 310 1.52 90.0 270 1.18 81.0 227 0.71 69.0 250 1.00 75.0 Control 206 0.76 0.20 mL, exposed with salmon oil only 304 1.52 0.30 mL, exposed with salmon oil only 184 0.70 0.18 mL, exposed with salmon oil only 3-Days 30 208 0.79 6.3 0.03 30 278 1.22 8.4 0.03 244 0.81 7.2 156 0.46 4.5 193 0.71 6.0 196 0.68 6.0 Control 253 0.89 0.25 mL, exposed with salmon oil only 272 1.23 0.27 mL, exposed with salmon oil only 233 0.75 0.23 mL, exposed with salmon oil only 7-Days 30 212‡ 0.71 6.3 0.03 30 121 0.30 3.6 0.03 230 0.76 6.9 241 0.88 7.2 204 0.74 6.0 167 0.53 5.1 Control 273 1.12 0.27 mL, exposed with salmon oil only 305 1.45 0.30 mL, exposed with salmon oil only 250 0.89 0.25 mL, exposed with salmon oil only

†Trout was found dead and the liver sample was not collected (NC). ‡Trout was found sick (not equilibrium condition) and the collected liver specimen was not used in this study for composite preparation. All control liver samples were mixed together to make one control composite specimen. The collected MX and or MK exposed liver samples were composited mixing three liver for each dosing level with exception of sick or dead fish liver. (Source: Reproduced with permission from (48), 2012, © Elsevier).

Table 3: In vivo trout exposure dosing schedule with nitro musk compounds and salmon oil vehicle.