Sr Plant Constituents Test Performed Aqueous Extract Methanol Extract
1 Carbohydrate Benedict test
Molisch test
+
+
+
+
2 Protein and Amino acids Ninhydin test
Biuret test
+
+
+
+
3 Fats and Fatty acids Phenophthalin test
Spot test
+
+
+
+
4 Alkaloid Mayer test
Wagner test
Dragondroff test
-
-
-
+
+
-
5 Terpenoid Salkovaski test + -
6 Steroid Liberman
Buchard test
+ +
7 Flavonoid Shinoda test
Alkaline
reagent test
+
-
+
-
8 Tannin and Phenolic compound Ferric chloride test
Lead acetate test
-
+
-
+
9 Saponin Froth test
Lead acetate test
+
+
+
+
10 Glycoside      
10a Cardiac glycoside Keller killani test + +
10b Anthraquinone glycoside Ammonia test + -
10c Coumarin glycoside Alkaline test + -
Table 2: Phyto-chemical screening of the Annona muricata leaf extract