Injection Retention Time Area Injection-1 4.451 34316 Injection-2 4.452 34431 Injection-3 4.454 34085 Injection-4 4.459 34350 Injection-5 4.452 34814 Injection-6 4.458 34360 Average 4.454 34392.7 Standard Deviation 0.003 237.7 %RSD 0.076 0.7

Table 2: Intra-day precision of the proposed method for Alogliptin in plasma.