Days Retention Time Area Day-1* 4.451 33218 Day-2* 4.456 33876 Day-3* 4.451 33790 Average 4.452 33628 Standard Deviation 0.003 358 %RSD 0.06 1.1

Table 4: Inter-day precision of the proposed method for Alogliptin (on three consecutive days n=6) in plasma. *Average of Six injections.