Figure 1: Time course of TI lesions. Ratio of kidney weight to body weight demonstrates kidney hypertrophy at days 21 and 35 (A). Urinary protein level indicates a rapid elevation in AA group as soon as day 3 (B). Urinary N-acetyl-ß-D-glucosaminidase (NAG) level in AA group shows a significant increase by day 3 and remains elevated during the 35 days period (C). Decreased urinary leucine aminopeptidase (LAP) level (D) attests tubular damage. Representative photographs of tissue injury (x200) at different time points: control condition (E); day 3 – increase of interstitial cells (F-arrow); day 7 - patchy zones of necrotic proximal tubular epithelial cells (PTECs) (#) associated with significant cell infiltration (arrow) (G); day 10-increase in necrotic PTECs (H); day 21 – increased tubular necrosis with appearance of atrophic tubules and significant cell infiltration (arrow)- (I); day 35 - extended zone of atrophic tubules (star) associated with infiltration of interstitial cells (arrow) - (J). Values are means ± SEM. N=6 in each group. *p ≤ 0.05 versus SHAM rat at corresponding time-point.