Figure 3: Typical morphology of 3D HUVEC cultures. Untreated (A), or in the presence of purified β2GPI (B) and the (C) Dimer; and (D) Monomer subfractions. Cultures were initiated with 1x104 cells seeded in 1mL RPMI 1640 medium supplemented with an antibiotic mixture around a 30 µL Matrigel® button, as described under materials and methods section. The β2GPI moieties (0.2 µM) were added into medium after cell adhesion and the growth was evaluated after 24h culture. Photomicrographies were taken in an inverted microscope under 10x magnification.