Figure 1: Immunohistochemical localization of ASIC2 in rat brain and spinal cord. In 4th ventricle (a and b), the immunoreactivities of ASIC2 are detected in ependymal cells (c) and choroid plexus (d). In ependymal cells, the reactions of ASIC2 are detected on cilia (arrows in b and c). In spinal cord (e), the reaction is detected in the marge of central canal (※ in f). Results with primary antibody against ASIC2 following preabsorption with the antigen are showed in lower 4 panels; 4th ventricle (g and h) and spinal cord (i and j). ASIC2 reactions are not detected in ependymal cells, choroid plexus (h) and central canal (※ in j). Bars; 500 μm (a, e, g and i), 50 μm (b and j) and 20 μm (c, d, f and h).