Figure 2: Quantitative analysis of each individual marker. A: Quantification of positive cells for each marker in control and tumor tissue samples, expressed as % of the total number of cells in the region of interest. Three markers were highly expressed in most of gliomas and absent in normal controls and less frequent in other tumors: nestin, SPARC and IDH1(R132H). B: General views of the TMA showing tissue samples represented by 5 sections each (when available). The comparison of the staining of the three selected markers shows co-expression of nestin and SPARC in numerous samples, whereas a smaller number of samples express IDH1(R132H). The non-tumoral control cases (F, E and G) are located in the frames.