miRNA detection
method |
Advantages |
Disadvantages |
| Northern blotting |
Detection without amplification, all forms of miRNAs can be detected. |
low-throughput, consuming time and large amount of RNA, hard to detect miRNAs with low copy numbers |
| qRT-PCR |
quick and easy to perform, relative low cost and small amounts of sample consummation |
amplification needed, generation of non-specific amplification products |
| Microarray |
high-throughput |
amplification needed, non-specific hybridization, not accurate and need to be confirmed |
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