Figure 2: Immunofluorescence analyses of E-cadherin and vimentin antibodies in breast cancer spheres from MCF7 and MDA-MB-231 cancer cell lines. MCF7 (A) and MDA-MB-231 (B) breast cancer spheres were stained using E-cadherin (E-cad) antibody, an epithelial marker. The expression of E-cadherin was significantly increased in MCF7 (A) and MDA-MB-231 (B) breast cancer spheres after treatment of TGF beta for 24 and 48 hours, compared to untreated breast cancer spheres. The positive staining of vimentin, a mesenchymal marker, was detected in both MCF7 and MDA-MB0231 breast cancer spheres. However, the expression of vimentin was not changed in breast cancer spheres in between untreated and treated with TGF beta during 24 and 48 hours. Ecadherin was stained with Alexa 488 green labeled and vimentin was stained with Alexa 568 red labeled secondary antibodies. Nuclei were stained with 4′,6′-diamidino-2-phenylindole (DAPI) (blue). Original magnification: 400X.