Figure 4: Effects of resveratrol on hepatic vascular endothelial growth factor (VEGF) expression during DENA-initiated hepatocarcinogenesis in female Sprague-Dawley rats. Rats were sacrifi ced 20 weeks following the commencement of the study and immunohistochemistry was performed to detect VEGF. Arrowheads indicate immunohistochemical staining of VEGF. (A) Near absence of immunopositivity in normal liver (100 ); (B) and (C) intense immunoreactivity in DENA control liver (100 and 250× respectively); (D) no alteration of expression in 50 mg/kg resveratrol group (100×); (E) decreased expression in 100 mg/kg resveratrol group (100×); and (F) near absence of VEGF expression in 300 mg/kg resveratrol group (100×) are noticed.