| Gene |
Exon |
Primer sequence |
Tm (C) |
Product size (bp) |
| EGFR |
18 |
F-5´CCAAATGAGCTGGCAAGTG 3'
R-5´TCCCAAACACTCAGTGAAACAAA 3' |
58 |
397 |
| 19 |
F-5´CCCAGTGTCCCTCACCTTC 3'
R-5´GCAGGGTCTAGAGCAGAGCA 3' |
62 |
306 |
| 20 |
F-5´CATTCATGCGTCTTCACCTG 3'
R- 5´CATATCCCCATGGCAAACTC 3' |
58 |
377 |
| 21 |
F-5´GCTCAGAGCCTGGCATGAA 3'
R-5´CATCCTCCCCTGCATGTGT 3' |
62 |
348 |
| PTEN |
5 |
F-5′GCAACATTTCTAAAGTTACCTACTTG 3'
R-5′CCAATAAATTCTCAGATCCAGG 3' |
50 |
378 |
| |
7 |
F-5′TGGTATGTATTTAACCATGC 3'
R-5′ CCTTATTTTGATATTTCTCCC 3' |
57 |
231 |
| |
8/1 |
F-5′TGCAAATGTTTAACATAGGTGA 3'
R-5′CCTTGTCATTATCTGCACGC 3' |
55 |
246 |
| |
8/2 |
F-5 GGAAGTCTATGTGATCAAGA 3'
R-5′ CGTAAACACTGCTTCGAAATA 3' |
53 |
286 |