Figure 3: De-adhesion dynamics of MDA-MB-231 cells on collagen-coated surfaces at varying densities. (A) Upon incubation with trypsin, cells were imaged every 3 seconds till they rounded up but remained attached to the substrate. Representative images of cell rounding on various surfaces. Scale bar=50 μm. (B) De-adhesion of the cells were quantified by plotting normalized area as a function of time. Mean de-adhesion curves (points) at different densities reveal faster de-adhesion at higher densities. The lines denote fit of the experimental curves with Botzmann equation. (C, D) Boltzmann fits to experimental curves yielded time constants τ1 and τ2 for each condition. Increase in collagen density led to reduction in both time constants. Bars indicate statistical significance (*p < 0.05).