Figure 5: Vascular morphogenesis and morphology were not altered on hypoxic ECM. (A) Immunofluorescence images of CLS formed from HUVECs cultured atop de-cellularized ECM from hypoxic and atmospheric-exposed NuFF/MDA231 co-cultures. Insets are high magnification of the boxed area. Scale bars are 50 μM for image insets. Phalloidin in green, CD31 in red and nuclei in blue. (B) Analyses of vascular morphological features including (i) vascular abundance, (ii) mean vascular branches, (iii) mean vascular diameters and (iv) maximum vascular diameters between structures grown on hypoxic and atmospheric ECM.