Figure 6: ROS modulators prevent down-regulation of Bcl-2 by PM. LNCaP and PC-3 cells were treated with PM in the presence or absence of NAC (100 mM), DPI (5 μM), ROT (5 μM) or MnTBAP (100 μM) for 20 h and cell lysates were analyzed for Bcl-2 and β-actin (loading control) by western blotting. Cells were also treated with LY83583 (10 μM) as a positive control in the presence or absence of MnTBAP. Bar graphs show relative expression of Bcl-2 compared to untreated control cells (Mean ± S.D.). Similar results were obtained in two independent experiments.