Residues Cnsw (7, 9) Cnsw (23, 24) Cnie (6, 10) Cnie (23, 24) Fibronectin binding Q49E -0.00082 0.14000 0.00043 0.22767   R130K -0.00006 0.11945 0.00081 0.22767   T142S 0.00079 0.08863 0.00038 0.22767   M193I 0.00278 0.15784 0.00323 0.22767 X N194K 0.00166 0.23084 0.00064 0.22767 X E204T 0.00253 0.25597 0.00271 0.22767 X I243V -0.00197 0.06348 -0.00074 0.22767   R249K -0.00161 0.11945 -0.00013 0.22767   D283E -0.00022 0.13905 0.00058 0.22767   S343A 0.00052 0.12061 0.00134 0.22767 X P345A 0.00190 0.29930 0.00307 0.22767 X T357A 0.00280 0.17341 0.00179 0.22767 X S388T -0.00322 0.08863 0.00059 0.22767   AUC 0.97619 0.95238 0.97619 0.50000   Threshold 0.00023 0.14892 0.00061     Sensitivity 0.85714 1.00000 0.85714     Specificity 1.00000 0.83333 1.00000

Table 2: Two conservation score sets with the first and second highest AUC of the variability based upon a substitution matrix and sequence weights and two conservation score sets with the highest AUC and between the 23rd and 24th nodes of the variability based upon information entropy in 276 (=24C2) conservation score sets, respectively. The “X” letter denotes the MpEF-Tu residue involving fibronectin binding [25]. The threshold shows the arithmetic mean between two conservation scores whose sum of the sensitivity and specificity becomes maximum when the threshold is used as a cutoff value of the conservation score set.