Figure 3: CTL-mediated lysis of HMEC in cytotoxicity assays. Nonactivated HMEC (nHMEC) and tumor-conditioned medium activated HMEC (aHMEC) (3.7 104 cells/well) were incubated with effector CTL at a ratio of 1:8 and were imaged using an inverted phase contrast microscope. Untreated nHMEC and aHMEC (a, a) are compared with nHMEC and aHMEC incubated with: CTL stimulated with control (unloaded with antigens) DC (b, b), CTL stimulated with DC loaded with aHMEC lysate (c, c), CTL stimulated with DC loaded with nHMEC lysate (d, d), CTL stimulated with DC loaded with FCSP prepared from aHMEC (e, e), and CTL stimulated with DC loaded with FCSP prepared from nHMEC (f, f). For panels a-f, nHMEC are the target cells, and in panels a-f, aHMEC are the target cells.