Figure 3: Protein and activity profi les of CDK2 in WISH cells treated with IFNγ. WISH cells were either not treated (control) or treated with 200 U of IFNγ for 24 hrs, 48 hrs or 72 hrs and the whole cell lysates were: western blotted for CDK2 (A), or assayed for total CDK2 activity (B) or cyclin E-CDK2 activity (C). The densitometry values (indicated below respective lanes) for the treated samples in the representative blots/assays were normalised with respect to the control. Standard error for multiple independent experiments was 10%. PS: Ponceau S stained gel representing equal loading.