| Name of the cell cycleregulated
protein |
Per cent level of the protein with respect
[Duration of treatment with IFNγ (hrs)] to control |
| |
[24] |
[48] |
[72] |
| CDK2 |
100 |
50 |
50 |
| Cyclin E |
50 |
10 |
20 |
| Cyclin A |
50 |
80 |
30 |
| Cyclin H |
90 |
40 |
50 |
| CDK7 |
80 |
120 |
140 |
| CDC25A |
120 |
90 |
50 |
| p21 |
60 |
40 |
10 |
| p27 |
80 |
50 |
02 |
| Cyclin D |
80 |
10 |
20 |
| CDK4 |
90 |
90 |
80 |
The total lysates from the cells, which were treated with IFNγ for different duration,
were fractionated on SDS-PAGE, western blotted for the cell cycle-regulated
proteins with respective antibody and the levels of the protein in the blots were
quantitated using ImageGauge version 2.54 |
