Figure 7: Expression of caspase-3 activity. PHO–S induced apoptosis in B16F10 tumor cells in caspase-3-dependent manner, was measured by flow cytometry. (A, B and C) Z-VAD-FMK nonspecific fluorescent control in B1610 tumor cell. Caspase-3 activity B16F10 tumor cells untreated (D), PHO-S treated B16F10 tumor cells (E and F). The results are representative of three separate experiments. Data are expressed as a dot plot of mean (±SD).