Figure 6: Tumor angiogenesis evaluation in CaneCPI-4-treated animals. B16F10-Nex2 cells (5x104) were injected subcutaneously in C57Bl/6 male in the presence of Cane-CPI-4 or PBS. When tumors in the control group reached a diameter of 1 cm, tumors were removed and fixed in formalin for immunohistochemistry analysis of angiogenesis by antibodies to CD34. (A) Quantification of microvessels. Error bars, SD of 5 animals. (B) Immunohistochemical analysis of CD34 expression in tumor tissue. (a) PBS control and (b) mice treated with CaneCPI-4 at 1 ÁM.