Figure 3: Samples were inactivated by heat-denaturation (70°C for 20 minutes) or digestion with ribonuclease A. Afterwards a standard containing 100 293T cells was spiked into every denaturated sample dilution and tRQ-TRAP assay was performed. There was no observable PCR inhibition independent of denaturation technique in all dilutions compared to the standard alone (depicted as dash line).