Figure 1: Effects of 10 μM ritonavir treatment on the proliferation and induction of apoptosis in MCL. Left Panel: proliferation of GP (A), GRL (B), and JVM2 (C) after 24, 48, or 72 hour incubation periods with 10 μM ritonavir, as assessed using MTT assays. GP, GRL, and JVM2 cell lines were seeded at 10,000 cells per well in a 96 well plate and incubated with 10 μM ritonavir for 24, 48, or 72 hours. Two hours prior to the given time point, 5 mg/ml MTT was added to each well, and cells were harvested at time point using a detergent-based buffer. Proliferation of the cells was assessed calorimetrically using a spectrophotometer. Right Panel: Induction of apoptosis in GP, GRL, and JVM2 after 48 or 72 hour incubation periods with 10 μM ritonavir, as assessed using AnnexinV:FITC apoptosis assay. GP, GRL, and JVM2 cells (1 x 106 cells) were treated with 10 μM ritonavir for 48 or 72 hours. The AnnexinV:FITC substrate and/or propidium iodide was then added to cells, and cells were analyzed via flow cytometry for positive staining. Cells positive for AnnexinV alone, or both AnnexinV and propidium iodide considered to be apoptotic.