Figure 4: Effect of co-administration of mitomycin C and cisplatin with or without the administration of B.aristata on the rate of on NADH dehydrogenase and succinate dehydrogenase of kidney mitochondria. The animals were divided into four groups of six animals each. Group-1, received saline, Group-2, received a single dose of mitomycin C (2 mg/kg body weight, i.p) and cisplatin (12 mg/kg body weight, i.p). Rats were sacrificed 48 h after the administration of drugs. Group-3, received alcoholic extract of B.aristata (10 mg/100 g body weight, oral) for three days. Group-4 recieved alcoholic extract of B.aristata (10 mg/100 g body weight, oral) for three days, followed by single dose of mitomycin C (2 mg/kg body weight, i.p) and cisplatin (12 mg/kg body weight, i.p). Rats were sacrificed 48 h after the administration of drugs. Values are mean S.D. from at least six rats in each group. NADH dehydrogenase assay: 20 g of mitochondrial protein was used for each assay NADH (10 mM) was used as substrate. NADH dehydrogenase activity was expressed as NADH units/min/ mg Protein (One NADH Unit = One n mole of potassium ferricyanide reduced /min/ mg / protein). Succinate dehydrogenase assay: 30 g of mitochondrial protein was used for each assay. Succinate (9 mM) was used as substrate. SDH activity was expressed as SDH units/ min/ mg protein (One SDH Unit + One n mole of DCPIP reduced/min/ mg protein). Results are expressed as relative to control which was taken as 100. The control values of NADH dehydrogenase and succinate dehydrogenase are 2030 + 214 and 107 + 14 respectively in liver mitochondria. Values are Mean SD for at least 13 animals. * P<0.05, **P < 0.01 and ***P< 0.001.