Figure 5: SF1126 potently inhibits phosphorylation of Akt and GSK-3. SUDHL-4 and TOLEDO cells were untreated or treated with SF1126 at 5 M, 10 M, 20 M, 40 M and 50 M for 1-hr. Cells were collected for protein isolation. 50 g of total protein from each lysate was resolved by SDS-PAGE and immunoblotted with antibodies specific for pan-Akt, phosphorylated Akt (Ser473) and phosphorylated GSK-3 (Ser9). -actin was used as a loading control.