Figure 4b: Effects of SETD4 knockdown on the viability of MDA-MB 231 cells, as measured by the MTT assay. Mock (non-transfected MDA-MB 231 cells), vector (cells transfected with empty vector) and two independent clones (shSETD4 3 and 4) were plated in medium containing FBS for 72 h. Mock and vector cells were also maintained in medium without FBS (0% serum). The results are expressed relative to mock cells. Means followed by the same letter indicate no significant difference by Tukey’s test (p<0.001). The results are representative of three independent experiments.