Figure 41: Effect of Nutlin on LNCAP cells treated with leptomycin and DR5. This graph depicts the effect of nutlin on combination of varying concentrations of leptomycin and DR5 on LNCAP cells. The LNCAP cells were plated at 10,000cells/ml in a 6 well plate and incubated for 24 hours. These were then treated with nutlin and incubated for 5 hours followed by addition of varying concentrations of leptomycin B, DR5 and their combinations and incubated for 24 hours. Images of cells were captured before and after incubation with drugs through light microscopy for calculating percentage apoptosis.