DRUG % Apoptosis
LM 0 nM 5.67
LM 0.5 nM 12
LM 1 nM 22
LM 5 nM 36.67
DR5 0 ng/ml 10
DR5 5 ng/ml 32
DR5 50 ng/ml 50.67
DR5 500 ng/ml 68.67
LM 1 + DR5 5 ng/ml 41.33
LM 5 + DR5 5 ng/ml 48.67
LM 1 + DR5 50 ng/ml 65.33
LM 5 + DR5 50 ng/ml 81.33
Table 1: Effect of leptomycin [LM], DR5 and their combined effects in an order of increasing concentrations on PC3 cells. The PC3 cells were plated at 50,000cells/ ml in a 24 well plate and were treated with varying concentrations of leptomycin B, DR5 and their combinations. After an incubation period of 24 hours, these cells were stained and counted using Hoechst stain by fluorescence microscopy.