Figure 3: Propolis/CAPE is an HDAC inhibitor and induces hyperacetylation of histone proteins: Protein lysates were obtained from cells were treated with CAPE or propolis for 24 h, or from PBMC from a healthy volunteer after a 3 week oral ingestion of CAPE-containing propolis. Western Blotting was performed for acetylated histone H3. Hyperacetylation of histone proteins is induced in a concentration-dependent manner by both CAPE and propolis (normalized for CAPE content) in MCF-7 and MDA-231 cells (Figure 3A) and in peripheral blood mononuclear cells from a healthy human volunteer after oral ingestion of CAPE-containing propolis (Figure 3B).