Forward primer 5´>3´ Reverse primer 5´>3´   GSTP1 M TTCGGGGTGTAGCGGCGTC GCCCCAATACTAAATCACGACG 57° U GATGTTTGGGGTGTAGTGGTTGTT CCACCCCAATACTAAATCACAACA P16 M TTATTAGAGGGTGGGGCGGATCGC GACCCCGAACCGCGACCGTAA 60° U TTATTAGAGGGTGGGGTGGATTGT CAACCCCAAACCACAACCATAA CyclinD2 M GGCGGATTTTATCGTAGTCG CTCCACGCTCGATCCTTCG 57° U AGAGTATGTGTTAGGGTTGATT ACATCCTCACCAACCCTCCA RAR 2 M GAACGCGAGCGATTCGAGT GACCAATCCAACCGAAACG 55° U GGATTGGGATGTTGAGAATGT CAACCAATCCAACCAAAACAA RASSF1A M GTTGGTATTCGTTGGGCGC GCACCACGTATACGTAACG 57° U GGTTGTATTTGGTTGGAGTG CTACAAACCTTTACACACAACA HIN-1 M GGTACGGGTTTTTTACGGTTCGTC AACTTCTTATACCCGATCCTCG 57° U GGTATGGGTTTTTTATGGTTTGTT CAAAACTTCTTATACCCAATCCTCA SOCS1 M1 GTT GTA GGA TGG GGT CGC GGT CGC CTA CTA ACC AAA CTA AAA TCC ACA 63° U1 GTT GTA GGA TGG GGT TGT GGT TGT CTA CTA ACC AAA CTA AAA TCC ACA M2 TTGTTCGGAGGTGGATTT ACTAAAACGCTACGAAACCG U2 TTTTTTCCTCTTCGTTTGGAGGTTGGATTTT AAAACAAAACAATAAACTAAAACACTACAAAACCA TWIST M TTT CGG ATG GGG TTG TTA TC AAA CGA CCT AAC CCG AAC G 56° U TTT GGA TGG GGT TGT TAT TGT CCT AAC CCA AAC AAC CAA CC TIMP3 M CGTTTCGTTATTTTTTGTTTTCGGTTTC CCGAAAACCCCGCCTCG 59° U TTTTGTTTTGTTATTTTTTGTTTTTGGTTTT CCCCCAAAAACCCCACCTCA DAPK M GGA TAG TCG GAT CGA GTT AAC GTC CCC TCC CAA ACG CCG A 57° U GGA GGA TAG TTG GAT TGA GTT AAT GTT CAA ATC CCT CCC AAA CAC CAA

Table 2: MSP-primers sequences for selected genes. M: Methylated; U: Unmethylated. Melting temperature are shown.