Figure 1: Downregulation of LFG and LEF-1 gene expression. A. The human breast carcinoma cell line MCF-7 were transfected with siRNA LEF-1 for 48 h and analysed for relative LEF-1 mRNA ratio compared to the control using RT-PCR, and B. MCF-7 cells were transfected with siRNA LFG for 48 h and analysed for relative LFG mRNA ratio compared to the control using RT-PCR. The data are the means ± SD of triplicate determinations which were repeated in three separate experiments. *p<0.05 vs. control. C. MCF-7 cells were transfected with siRNA (a: siRNA LEF-1 or siRNA LFG; b: siRNAcontrol; c: nontransfected cells ) for 48 h and then cell lysates were subjected to Western blotting to detect the quantity of expressed LEF-1 (LFG). Actin was used for normalization.