Figure 4: The impact of LFG suppression on the apoptosis of MCF-7 cells after chemotherapeutical treatment with “Erlotinib” and “Trastzumab”. A. MCF-7 breast cancer cell was transfected with siRNA LFG. Following transfection the cells were treated with the chemotherapeutic agent “Erlotinib” using different times (2h; 4h) and concentrations (0,25mg/ml; 0,5mg/ml; 1mg/ ml). These stimulated cells were analysed for activated level of caspase 3 using the Apo-One assay and compared to the nontransfected cells and the control. B. MCF-7 breast cancer cell were transfected with siRNA LFG. Following transfection the cells were treated with the chemotherapeutic agent “Trastzumab” using different times (2h; 4h) and concentrations (0,25mg/ml; 0,5mg/ml; 1mg/ml). These stimulated cells were analysed for activated level of caspase 3 using the Caspase Assay and compared to the nontransfected cells and the control. Data are the means ± SD of duplicate determinations which were repeated in two separate experiments. *p<0.05, **p<0.01, ***p<0.001 vs. the control.