Figure 5: Effect of STAT1 inhibition on cell motility with IL-27 and apricoxib combination. (A) After treatment of A549 cells with IL-27 (50 ng/mL) at 60-70% confluence, a scratch was made in a monolayer of cells and the closure of the wound gap was observed under the microscope (50x magnification) up to 2 days. (B) The cells were treated with STAT1 or a non-targeting control siRNA (40 nM) for 6 hours and then pretreated with apricoxib (0.016-2 μM) for 4 hours followed by exposure to IL-27. Results show the cell migration at 2 days after IL-27 treatment. Images were analyzed and % area invaded was measured as described in Materials and Methods.