Figure 3: Expression of cytokine genes in THP-1. After THP-1 cells had been cultured in RPMI-1640 media at pH 7.5 or 6.7 for 2 days with simvastatin at the concentrations indicated, the amounts of indicated mRNA were measured as described in Materials and Methods. The left column: Relative increases in mRNA levels of TNF-α (A), IL-1β (C), and IL-8 (E) are represented. The right column: Copy numbers of mRNA per cell of TNF-α (B), IL-1β (D), and IL-8 (F) are represented. The mean values and S.D. obtained from three experiments using different cultures are represented. The p values compared with data obtained without simvastatin at both pH values were calculated. *p< 0.01; no mark, p>0.01. The p values at pH 6.7 compared with data at pH 7.5 were calculated in the absence of simvastatin. **p< 0.01.