BRUCE
Figure 3: BRUCE knockdown sensitizes resistant MDA-MB-231 cells to cisplatin and taxol-induced cell death. (A) Western blot of BRUCE protein levels preand post-lentiviral knockdown for 48 hrs in MDA-MB-231 cells exposed to lentivirus expressing five BRUCE-targeted shRNA (#7 - #11) and a scramble control (ctrl). (B) MTT assay of MDA-MB-231, MDA-MB-468, and MCF-7 cell lines following 1-5 days of lentiviral exposure (shRNA #9). Result of three independent experiments +/- SEM. (C) MDA-MB-231 cells treated with control or shBRUCE lentivirus for two days were exposed to increasing concentrations of cisplatin (0-10 μM) for three days and cell viability was measured by MTT assay. (D) MDA-MB-231 cells were exposed to shBRUCE lentivirus for two days followed by treatment with cisplatin (5 μM) or taxol (0.65 μM) for the indicated time points. Following treatment, the MTT assay for cell viability was performed. (E) MDA-MB-231 cells exposed to shBRUCE lentivirus for two days were co-exposed to cisplatin (2.5 μM) and taxol (0.65 μM) for the indicated time points. Following treatment the MTT assay for cell viability was performed. Result of three independent experiments +/- SEM.