metabolic
Figure 2: Study of the metabolic activity of L. fermentum NCIMB 5221: Determination of growth pattern and total free fatty acid (FFA) profile. The concentrations of total FFAs (μM PAE) in the probiotic bacterial culture of L. fermentum NCIMB 5221 was determined during the (a) lag and exponential, (b) stationary, and (c) death phases of bacterial growth. L. acidophilus ATCC 314 and L. rhamnosus ATCC 53103 were used as controls. (d) Variation of total FFAs levels per viable bacterial cell for the exponential and stationary phases. (e) Description of the levels of total FFA per gram of bacterial pellet at 12 h and 14 h of growth. All these features of L. fermentum NCIMB 5221 were compared to L. acidophilus ATCC 314 and L. rhamnosus ATCC 53103 at the same conditions, for 32 h in MRS broth (37oC, 5 % CO2) *p < 0.05, **p < 0.01 and ***p < 0.001 compared to L. acidophilus ATCC 314 and L. rhamnosus ATCC 53103. Data represent the mean ± SEM (n=3). PAE: Palmetic acid equivalents. L. a 314: L. acidophilus ATCC 314; L. rh 53103: L. rhamnosus ATCC 53103; L. f 5221: L. fermentum NCIMB 5221.