Groups Age range %AF+ig/ %AF+ tc Sex fem/masc Types and concentration (ng/mL) of aflatoxins excreted in urine, (number of cases) HPLC ngAF/mg cr (N° +  cases) ELISA ngAF/mg cr (N°+ cases)
AFB1 AFM1 AFP1 AFB-Gua
Risk groups
HBV 26-68 50/7 11/19 170 - 324 (3) 2 - 90 (4) 20 - 36 (3) 1 - 43 (5) 1 - 590 (6) 0.1 - 1543 (15)
HCV 34-64 17/2 19/11 0 1 - 44 (4) 0 1 - 4 (4) 2 - 63 (7) 3 - 60                (8)
VC 30-72 27/4 18/12 423 - 502 (2) 14  - 101 (5) 48 - 127 (2) 0.4 - 323 (6) 0.3 - 1693 (11) 0 – 4106 (15)
Range 26-72 10-50% 48/42 170 - 502 1 - 101 20 - 127 0.4  – 323 0.3 -1693 0.1 - 4562
N° positive cases 5/210 (2.4%) 13/210 (6.2%) 5/210 (2.4%) 15/210 (7.2%) 24/210 (11.4%) 38/210 (18.1%)
Control groups
AC 41-74 10/1 10/20 0 0 0 1 - 2 (3) 0.3 - 3 (2) 1 - 10 (3)
KF 35-71 3/1 17/13 0 0 0 0 0 2
CNHD 34-69 0/0 19/11 0 0 0 0 0 0
H 26-72 10/1 15/15 0 1 - 4 (3) 2 - 3 (2) 1 (1) 1 - 10 (4) 9 - 42 (6)
Total 26-74   109/101            
N° positive cases 0 3/210 (1.4%) 2/210 (1.0%) 4/210 (1.9%) 6/210 (2.9%) 9/210 (4.3%)
Statistical analyses
P value in F test* 0.036 0.0532 0.0013 0.277    
Total number of 47 positive cases in  210 total number of samples 5/210 (2.4%) 16/210 (76.2%) 7/210 (3.3%) 19/210 (9.1%)    
P values in contrastsa VC vs HBV : 0.036 VC vs H: 0.019 VC+HBV+ HCV vs H : 0.029 VC vs H : 0.002 HBV vs H :0.023 VC+HBV vs H: 0.0005 Non-significant at 0.05%    
HBV : Viral B Hepatitis; HCV: Viral C Hepatitis; VC:Viral cirrhosis; AC:Alcoholic cirrhosis; KF:Kidney failure. CNHD: Chronic non hepatic diseases; % AF + ig/ % AF + tc total cases: Percentage of aflatoxin positive cases inside the group of 30, between aflatoxin positive cases inside total 210 cases. *With data previously transformed using Box & Cox method. a: Always lower averages in Healthy (H). (N° + cases): Number of positive cases
Table 1: Excretion of AF types in hepatic diseases, by HPLC and ELISA methods, and statistical analysis.