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Figure 1: A: Percentage of adhering cells on: silicate granules (indicated with square), DMB (indicated with circle) and matrigel (indicated with triangle) measured by fluorescence intensity at t=30min, t=1h, t=4h and t=8h of culture times. Statistically significant differences were evident among biomaterials such **P<0.001 silicate granules versus matrigel coated plates and *P<0.05 silicate granules versus demineralized bovine bone (DMB). All experiments were performed in triplicated. B: metabolic activity of adhering BMMCs over the time on different surfaces. BMMCs seeded on silicate granules and DMB were cultured over 14 days. Percentage of adhering cells on silicate granules was determined at day 2, day 7 and day 14 (white bars). Number of adhering cells on DMB was determined at same days and indicated with light grey bars. C: Typical growth conformation of stem cells at low density (unit formation colony). |