Figure 6: Effect of RA on EGF-induced cell growth and migration. Cells were pretreated with RA for 30 minutes and then stimulated with EGF for 24 hrs. UM-SCC-6 (A) and UM-SCC-10B (B) cell growth was determined by measuring absorbance at 460 nm. Data are expressed as mean ± SD (n=5). *p<0.05, **p<0.01, ***p<0.001 vs. control. †††p<0.001, ††††p<0.001 vs EGF. To determine the effect of RA on migration, cells were pretreated with RA for 30 minutes, stimulated with EGF and migration was analyzed at 48 hrs. for UM-SCC-6 (C) and 72 hrs. for UM-SCC-10B cells (D). Data are expressed mean ± SD (n = 6). †p<0.05, ††††p<0.001 vs EGF.