A)   Cq±SD CTCF pCI Cq± SD EV pCI Cq change Fold change CTCF 17.005 ±0.0495 21.015 ± 0.0353 4.01 16.11 ERα 21.5 ±0.071 20.78 ± 0.0283 -0.72 1.65 GAPDH 17.425±0.106 17.175 ± 0.064 -0.25   TBP 23.34 ±0.14 22.745 ± 0.191 -0.59

B)   Cq±SD CTCF siRNA Cq±SD Non target siRNA Cq change Fold change CTCF 24.06 ± 0.014 21.945 ± 0.078 -2.11 4.32 ERα 20.835 ± 0.219 21.005 ± 0.007 0.17 1.13 GAPDH 18.45 ± 0.1697 18.52 ± 0.0567 0.07   TBP 23.68 ± 0.106 23.455 ± 0.0778 -0.22

Table 1: ERα mRNA fold change on CTCF overexpression and siRNA knockdown CTCF was overexpressed in MCF7 cells with a CTCF expression vector (CTCF pCI) and control empty vector (EV) pCI (A) and underwent knockdown on transfection with CTCF siRNA and a control EV siRNA (B). cDNA was reverse transcribed from 1 μg of extracted RNA and subjected to QPCR. Values are the average of two experiments, performed in duplicate. Table shows Cq levels for mRNA expression and fold changes for CTCF overexpression (A) and CTCF knockdown (B). Also shown are Cq levels for mRNA expression and fold changes for ERα and for reference genes GAPDH and TBP. A Cq change of less than 0.8 is accepted as ‘no difference’ in gene expression levels. The result indicates no significant change in ERα mRNA expression with significant fold changes in CTCF mRNA expression.