Figure 4: Effect of NAC pretreatment on apoptosis in MDA-MB231 cells treated with leinamycin. The cells were pretreated with 2 mM NAC for 24 h before exposure to various concentrations of leinamycin (0, 6.25, 25, and 50 ng/mL) for 24 h. Data are means ± SEMs of three separate experiments.