Figure 4: Cellular GSH. Representative HPLC runs of forebrain acid-soluble supernatants, showing the GS-bimane peak with a retention time of 14.3 min. Specimens were placed in the mBBr labeling reaction immediately after tissue collection. Panel A: Two specimens from untreated mouse; one sample was immediately incubated with mBBr and one was pre-incubated at 25°C in 10 mM NEM for 5 min before mBBr labeling. The calculation of cellular GSH (168 pmol mg-1) was corrected for peak present in the NEM reaction. Panel B: Representative HPLC runs of treated (19.2 mg) and untreated (17.0 mg) specimens. NEM (0.1 mM) and GSH standard (200 pmol) runs are also shown.