Btae TX-I (PLA2)
HPLC Fraction Measured Mass (Da) Amino acid sequence Theorical Mass (Da)
1 1377.7115 DL/IWQ/KFGQ/KMI/LL/IK/Q 1377.6687
2 2599.1301 L/IPFPYYTTYGCYCGWGGQ/KGQ/KPK/Q 2599.1233
3 1504.5356 CCFVHDCCYGK/Q 1504.4954
4 1661.7389 ENGVI/LI/LCGEGTPCEK/Q 1661.6716
5   951.3790 Q/KI/LCECDK/Q   951.3886
6   793.3905 AAAVCFR   793.3890
7 1414.6737 RYMAYPDVL/ICK/Q 1414.6905
8   975.4549 I/LDSYTYSK/Q   975.4623
Table 2: Measured molecular masses and deduced amino acid sequences obtained by ESI-MS/MS based on the alkylated tryptic peptides of Btae TX-I. The peptides were separated by RP-HPLC and sequenced by mass spectrometry. C = alkylated cysteine, lysine residues shown in bold were deduced on the cleavage and missed cleavage by trypsin. All molecular masses are reported as monoisotopic.